Journal of Nutraceuticals and Food Science

Beneficial effect of Gremin, a proprietary dual antioxidant formulation on skeletal muscle injury - in-vivo

Background: Tissue repair capabilities in mammals are relatively limited, and there is a growing interest to discover newer therapeutic interventions for the muscle regeneration process. While Curcumin and Green Coffee extract has previously shown to have beneficial effect individually, there is no study to examine the synergistic effects of these compounds. We, therefore, attempted to study the effect of Gremin, a proprietary encapsulated ingredient formulation in a mouse model with BaCl₂ induced muscle injury.

Methods and Findings: Adult male Swiss Albino mice were divided into four groups (n=6 per group) as vehicle control, BaCl₂ control, Gremin and reference compound group. Animals were administered with BaCl₂ injection to induce muscle injury, except for vehicle control. Gremin and the reference compound was administered at a dose level of 200 mg/kg/b.w daily, for up to 17 days via the oral route. Biochemical markers viz., TNF-α, Creatine Kinase (CK), Lactate Dehydrogenase (LDH), and histopathology for necrosis, were assessed.

Results: Histopathology analysis of the mouse muscle tissues demonstrated significant protective effects of Gremin on muscle injury. Gremin group exhibited reduced levels of LDH and CK enzyme biomarkers indicating muscle recovery.

Conclusion: Gremin Supplementation has beneficial effects on muscle injury and could be of immense value during post-injury rehabilitation.

Author(s):

Shankaranarayanan Jeyakodi*, Arunkanth Krishnakumar, Dinesh Kumar Chellappan, Rajesh Subbanna, Sachin Bansal

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